講演詳細

微生物部門[Forest Microbes]

日付	ポスター発表
会場名	（学生ポスター賞の審査対象）
講演番号	P-430
発表題目	Exploring the functions of miR164c/172a/319a/396a in the ectomycorrhizal formation of Populous tomentosa Exploring the functions of miR164c/172a/319a/396a in the ectomycorrhizal formation of Populous tomentosa
要旨本文	MicroRNAs (miRNAs) play significant roles in regulatory mechanisms in mycorrhizal symbiosis. In the previous study, we identified the differentially expressed miRNAs (DE-miRNA) in roots of Populus tomentosa inoculated by Cenococcum geophilum by miR-seq. In this research, we selected four DE-miRNAs (miR164c, miR172a, miR319a and miR396a) as candidate miRNAs to reveal how miRNAs regulate ectomycorrhizal formation. We constructed the overexpressed, knocked-down and knocked-out transgenic mutants of P. tomemtosa for these four DE-miRNAs. At present, the overexpressed and knocked-down transgenic mutant lines were successfully regenerated. The knocked-out mutants of P. tomentosa by CRISPR/Cas9 system are being prepared. As the next step, we will investigate the regulating mechanisms of four DE-miRNAs by using the constructed transgenic mutants.
著者氏名	○Tao, Yuanxun1 ・ Li, Chaofeng2 ・ Xu, Changzheng3 ・ Liu, Ying1 ・ Okabe, Shin1 ・ Lian, Chunlan2
著者所属	1The University of Tokyo ・ 2The University of Tokyo ・ 3College of Life Science, Southwestern University
キーワード	miRNA, ectomycorrhizal, Populus tomentosa
Key word	miRNA, ectomycorrhizal, Populus tomentosa