第136回日本森林学会大会 発表検索
講演詳細
微生物部門[Forest Microbes]
| 日付 | 2025年3月22日 |
|---|---|
| 開始時刻 | 9:15 |
| 会場名 | S32 |
| 講演番号 | M-2 |
| 発表題目 | ポプラにおける外生菌根形成を制御するmicroRNA319aの分子機構の解明 Exploring the functions and molecular mechanism of micro319a regulating ectomycorrhizal formation in Populus tomentosa |
| 所属 | 東京大学 |
| 要旨本文 | Ectomycorrhizal (ECM) symbiosis significantly impacts plant growth and development. This study highlights the regulatory role of Pto-miR319a in ECM formation in Populus tomentosa. Transgenic poplar lines with overexpressed (OE) or knockdown (STTM) Pto-miR319a were inoculated with Cenococcum geophilum (Cg). Colonization rates varied: OE lines had reduced colonization (21.1% ±0.13) compared to wild-type (52.9% ±0.09), while STTM lines showed increased colonization (77.3% ±0.03).Further analysis identified that Pto-miR319a targets Pto-TCP4 gene, and overexpression of Pto-TCP4 enhanced colonization (66.2% ±0.07), indicating that the Pto-miR319a-Pto-TCP4 pathway is essential in ECM development. These results offer novel insights into miRNA-mediated regulation of ECM symbiosis in poplar. |
| 著者氏名 | ○Chunlan Lian1 ・ Yuanxun Tao1 ・ Chaofeng Li1,2 ・ Ziwei Yang1 ・ Norihisa Matsushita1 ・ Changzheng Xu2 ・ Daisuke Tsugama1 |
| 著者所属 | 1東京大学大学院農学生命科学研究科 ・ 2西南大学 |
| キーワード | マイクロRNA, 外生菌根菌, ポプラ |
| Key word | miRNA, ectomycorrhizal fungus, Poplar |